| Name | City | State | Division | Region |
|---|---|---|---|---|
| University of Montevallo | Montevallo | Alabama | NCAA II | South |
| Harding University | Searcy | Arkansas | NCAA II | South |
| Ouachita Baptist University | Arkadelphia | Arkansas | NCAA II | South |
| Azusa Pacific University | Azusa | California | NCAA II | West |
| Biola University | La Mirada | California | NCAA II | West |
| Cal State Dominguez Hills | Carson | California | NCAA II | West |
| California State University Monterey Bay | Seaside | California | NCAA II | West |
| California State University Stanislaus | Turlock | California | NCAA II | West |
| Concordia University | Irvine | California | NCAA II | West |
| Point Loma Nazarene University | San Diego | California | NCAA II | West |
| Post University | Waterbury | Connecticut | NCAA II | Northeast |
| University of New Haven | West Haven | Connecticut | NCAA II | Northeast |
| University of the District of Columbia | Washington | District of Columbia | NCAA II | South |
| Nova Southeastern University | Davie | Florida | NCAA II | South |
| Albany State University | Albany | Georgia | NCAA II | South |
| Augusta University | Augusta | Georgia | NCAA II | South |
| Clark Atlanta University | Atlanta | Georgia | NCAA II | South |
| Clayton State University | Morrow | Georgia | NCAA II | South |
| Fort Valley State University | Fort Valley | Georgia | NCAA II | South |
| Paine College | Augusta | Georgia | NCAA II | South |
| Quincy University | Quincy | Illinois | NCAA II | Midwest |
| University of Indianapolis | Indianapolis | Indiana | NCAA II | Midwest |
| Newman University | Wichita | Kansas | NCAA II | Midwest |
| Bentley University | Waltham | Massachusetts | NCAA II | Northeast |
| Davenport University | Grand Rapids | Michigan | NCAA II | Midwest |
| Concordia University | Saint Paul | Minnesota | NCAA II | Midwest |
| Southwest Minnesota State University | Marshall | Minnesota | NCAA II | Midwest |
| Southwest Baptist University | Bolivar | Missouri | NCAA II | Midwest |
| University of Nebraska-Kearney | Kearney | Nebraska | NCAA II | Midwest |
| Wayne State College | Wayne | Nebraska | NCAA II | Midwest |
| Adelphi University | Garden City | New York | NCAA II | Northeast |
| Daemen College | Amherst | New York | NCAA II | Northeast |
| Long Island University-Post Campus | Greenvale | New York | NCAA II | Northeast |
| Molloy College | Rockville Centre | New York | NCAA II | Northeast |
| Pace University | Pleasantville | New York | NCAA II | Northeast |
| St Thomas Aquinas College | Sparkill | New York | NCAA II | Northeast |
| Belmont Abbey College | Belmont | North Carolina | NCAA II | South |
| Gardner-Webb University | Boiling Springs | North Carolina | NCAA II | South |
| Lees-McRae College | Banner Elk | North Carolina | NCAA II | South |
| Livingstone College | Salisbury | North Carolina | NCAA II | South |
| Saint Augustine’s University | Raleigh | North Carolina | NCAA II | South |
| Ashland University | Ashland | Ohio | NCAA II | Midwest |
| Malone University | Canton | Ohio | NCAA II | Midwest |
| Notre Dame College | Cleveland | Ohio | NCAA II | Midwest |
| University of Findlay | Findlay | Ohio | NCAA II | Midwest |
| Northeastern State University | Tahlequah | Oklahoma | NCAA II | South |
| Oklahoma Christian University | Oklahoma City | Oklahoma | NCAA II | South |
| Bloomsburg University | Bloomsburg | Pennsylvania | NCAA II | Northeast |
| Cheyney University of Pennsylvania | Cheyney | Pennsylvania | NCAA II | Northeast |
| Clarion University of Pennsylvania | Clarion | Pennsylvania | NCAA II | Northeast |
| Edinboro University of Pennsylvania | Edinboro | Pennsylvania | NCAA II | Northeast |
| Lincoln University | Lincoln University | Pennsylvania | NCAA II | Northeast |
| University of Pittsburgh Johnstown | Johnstown | Pennsylvania | NCAA II | Northeast |
| Claflin University | Orangeburg | South Carolina | NCAA II | South |
| Francis Marion University | Florence | South Carolina | NCAA II | South |
| Limestone College | Gaffney | South Carolina | NCAA II | South |
| University of South Carolina at Aiken | Aiken | South Carolina | NCAA II | South |
| Augustana University | Sioux Falls | South Dakota | NCAA II | Midwest |
| Northern State University | Aberdeen | South Dakota | NCAA II | Midwest |
| King University | Bristol | Tennessee | NCAA II | South |
| Le Moyne-Owen College | Memphis | Tennessee | NCAA II | South |
| Lincoln Memorial University | Harrogate | Tennessee | NCAA II | South |
| Dallas Baptist University | Dallas | Texas | NCAA II | South |
| Lubbock Christian University | Lubbock | Texas | NCAA II | South |
| Texas A & M International University | Laredo | Texas | NCAA II | South |
| Central Washington University | Ellensburg | Washington | NCAA II | West |
| Alderson Broaddus University | Philippi | West Virginia | NCAA II | South |
| Glenville State College | Glenville | West Virginia | NCAA II | South |
| Shepherd University | Shepherdstown | West Virginia | NCAA II | South |
| West Liberty University | West Liberty | West Virginia | NCAA II | South |
There are 70 NCAA Division 2 colleges that have Women's Cheerleading teams in the United States.