| Name | City | State | Division | Region |
|---|---|---|---|---|
| ASA College - Miami | Hialeah | Florida | NJCAA | South |
| Ave Maria University | Ave Maria | Florida | NAIA | South |
| Barry University | Miami Shores | Florida | NCAA II | South |
| Bethune-Cookman University | Daytona Beach | Florida | NCAA I | South |
| Eastern Florida State College | Melbourne | Florida | NJCAA | South |
| Eckerd College | Saint Petersburg | Florida | NCAA II | South |
| Embry-Riddle University | Daytona Beach | Florida | NCAA II | South |
| Flagler College | Saint Augustine | Florida | NCAA II | South |
| Florida Atlantic University | Boca Raton | Florida | NCAA I | South |
| Florida Gulf Coast University | Fort Myers | Florida | NCAA I | South |
| Florida National University | Hialeah | Florida | USCAA | South |
| Florida Southern College | Lakeland | Florida | NCAA II | South |
| Florida State University | Tallahassee | Florida | NCAA I | South |
| Keiser University | West Palm Beach | Florida | NAIA | South |
| Lynn University | Boca Raton | Florida | NCAA II | South |
| Palm Beach Atlantic University | West Palm Beach | Florida | NCAA II | South |
| Rollins College | Winter Park | Florida | NCAA II | South |
| Saint Leo University | Saint Leo | Florida | NCAA II | South |
| Southeastern University | Lakeland | Florida | NAIA | South |
| St Thomas University | Miami Gardens | Florida | NAIA | South |
| Stetson University | Deland | Florida | NCAA I | South |
| University of Central Florida | Orlando | Florida | NCAA I | South |
| University of Florida | Gainesville | Florida | NCAA I | South |
| University of Miami | Coral Gables | Florida | NCAA I | South |
| University of North Florida | Jacksonville | Florida | NCAA I | South |
| University of South Florida | Tampa | Florida | NCAA I | South |
| University of West Florida | Pensacola | Florida | NCAA II | South |
| Warner University | Lake Wales | Florida | NAIA | South |
| Webber International University | Babson Park | Florida | NAIA | South |