| Name | City | State | Division | Region |
|---|---|---|---|---|
| Averett University | Danville | Virginia | NCAA III | South |
| Bridgewater College | Bridgewater | Virginia | NCAA III | South |
| Christopher Newport University | Newport News | Virginia | NCAA III | South |
| Ferrum College | Ferrum | Virginia | NCAA III | South |
| Hampden-Sydney College | Hampden Sydney | Virginia | NCAA III | South |
| Hampton University | Hampton | Virginia | NCAA I | South |
| Marymount University | Arlington | Virginia | NCAA III | South |
| Randolph College | Lynchburg | Virginia | NCAA III | South |
| Randolph-Macon College | Ashland | Virginia | NCAA III | South |
| Roanoke College | Salem | Virginia | NCAA III | South |
| Shenandoah University | Winchester | Virginia | NCAA III | South |
| Southern Virginia University | Buena Vista | Virginia | NCAA III | South |
| University of Lynchburg | Lynchburg | Virginia | NCAA III | South |
| University of Mary Washington | Fredericksburg | Virginia | NCAA III | South |
| University of Richmond | Richmond | Virginia | NCAA I | South |
| University of Virginia | Charlottesville | Virginia | NCAA I | South |
| Virginia Military Institute | Lexington | Virginia | NCAA I | South |
| Virginia Wesleyan University | Virginia Beach | Virginia | NCAA III | South |
| Washington & Lee University | Lexington | Virginia | NCAA III | South |