| Name | City | State | Division | Region |
|---|---|---|---|---|
| Averett University | Danville | Virginia | NCAA III | South |
| Bluefield College | Bluefield | Virginia | NAIA | South |
| Bridgewater College | Bridgewater | Virginia | NCAA III | South |
| Bryant & Stratton College-Virginia | Virginia Beach | Virginia | NJCAA | South |
| Christopher Newport University | Newport News | Virginia | NCAA III | South |
| Eastern Mennonite University | Harrisonburg | Virginia | NCAA III | South |
| Emory & Henry College | Emory | Virginia | NCAA III | South |
| Ferrum College | Ferrum | Virginia | NCAA III | South |
| George Mason University | Fairfax | Virginia | NCAA I | South |
| Hampden-Sydney College | Hampden Sydney | Virginia | NCAA III | South |
| James Madison University | Harrisonburg | Virginia | NCAA I | South |
| Liberty University | Lynchburg | Virginia | NCAA I | South |
| Longwood University | Farmville | Virginia | NCAA I | South |
| Mary Baldwin University | Staunton | Virginia | NCAA III | South |
| Marymount University | Arlington | Virginia | NCAA III | South |
| Norfolk State University | Norfolk | Virginia | NCAA I | South |
| Old Dominion University | Norfolk | Virginia | NCAA I | South |
| Paul D Camp Community College | Franklin | Virginia | NJCAA | South |
| Radford University | Radford | Virginia | NCAA I | South |
| Randolph-Macon College | Ashland | Virginia | NCAA III | South |
| Roanoke College | Salem | Virginia | NCAA III | South |
| Shenandoah University | Winchester | Virginia | NCAA III | South |
| Southern Virginia University | Buena Vista | Virginia | NCAA III | South |
| Southwest Virginia Community College | Cedar Bluff | Virginia | NJCAA | South |
| The Apprentice School | Newport News | Virginia | USCAA | South |
| Thomas Nelson Community College-Hampton Campus | Hampton | Virginia | NJCAA | South |
| University of Lynchburg | Lynchburg | Virginia | NCAA III | South |
| University of Mary Washington | Fredericksburg | Virginia | NCAA III | South |
| University of Richmond | Richmond | Virginia | NCAA I | South |
| University of Virginia | Charlottesville | Virginia | NCAA I | South |
| University of Virginia's College at Wise | Wise | Virginia | NCAA II | South |
| Virginia Commonwealth University | Richmond | Virginia | NCAA I | South |
| Virginia Military Institute | Lexington | Virginia | NCAA I | South |
| Virginia State University | Petersburg | Virginia | NCAA II | South |
| Virginia Tech | Blacksburg | Virginia | NCAA I | South |
| Virginia Wesleyan University | Virginia Beach | Virginia | NCAA III | South |
| Washington & Lee University | Lexington | Virginia | NCAA III | South |
| William & Mary | Williamsburg | Virginia | NCAA I | South |