| Name | City | State | Division | Region |
|---|---|---|---|---|
| Alice Lloyd College | Pippa Passes | Kentucky | NAIA | South |
| Asbury University | Wilmore | Kentucky | NAIA | South |
| Bellarmine University | Louisville | Kentucky | NCAA I | South |
| Berea College | Berea | Kentucky | NCAA III | South |
| Brescia University | Owensboro | Kentucky | NAIA | South |
| Campbellsville University | Campbellsville | Kentucky | NAIA | South |
| Centre College | Danville | Kentucky | NCAA III | South |
| Eastern Kentucky University | Richmond | Kentucky | NCAA I | South |
| Georgetown College | Georgetown | Kentucky | NAIA | South |
| Kentucky Christian University | Grayson | Kentucky | NAIA | South |
| Kentucky State University | Frankfort | Kentucky | NCAA II | South |
| Kentucky Wesleyan College | Owensboro | Kentucky | NCAA II | South |
| Midway University | Midway | Kentucky | NAIA | South |
| Morehead State University | Morehead | Kentucky | NCAA I | South |
| Murray State University | Murray | Kentucky | NCAA I | South |
| Northern Kentucky University | Highland Heights | Kentucky | NCAA I | South |
| Spalding University | Louisville | Kentucky | NCAA III | South |
| Thomas More University | Crestview Hills | Kentucky | NAIA | South |
| Transylvania University | Lexington | Kentucky | NCAA III | South |
| Union College | Barbourville | Kentucky | NAIA | South |
| University of Kentucky | Lexington | Kentucky | NCAA I | South |
| University of Pikeville | Pikeville | Kentucky | NAIA | South |
| University of the Cumberlands | Williamsburg | Kentucky | NAIA | South |
| Western Kentucky University | Bowling Green | Kentucky | NCAA I | South |